Automated analysis in flow cytometry

P-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

immunoClust - Automated Pipeline for Population Detection in Flow Cytometry

Rare-event analysis in flow cytometry.

Technical aspects of rare-event detection are discussed in this article in a practical context, with two real-life examples. A growing number of flow cytometry-based assays depend on rare-event detection for basic science and clinical applications.

Quantification of cellular viability by automated microscopy and flow cytometry

Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342. However, a fraction of cells that exclude DAPI or exhibit normal nuclear morphology have already lost mitochondrial functions and/or manifest ...

Technology assessment of automated urine particle flow cytometry

The examination of the urine remains one of the most commonly performed tests in clinical laboratory practice. For over a century, standard microscopy analysis of urine has been considered as a golden standard. However, critical evaluation of sediment analysis has revealed a number of significant flaws, particularly in relation to centrifugation, the transfer error from the centrifugation tube ...